Thistle lost out 3-2 to Rob Roy in a dramatic opener to this season.
Largs Thistle have been drawn at home to Kirkintilloch Rob Roy in the New Coins Holdings West of Scotland Cup round 3.
The winners will face an away tie at either Cumnock or Blantyre Vics.
The Barrfields side have suffered two narrow defeats to Rob Roy in the Premier League this season, and will hope to make it third time lucky.
The last time both sides crossed swords in the tournament was during a dramatic 2010 semi-final when Thistle lost out in a dramatic penalty shoot-out at Kirkintilloch.
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